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Workshop on Microbiome Data Analysis
Workshop Title: Amplicon Sequencing (16S/18S/ITS) & Shotgun Metagenomics Data Analysis
Organized by: Negenome Bio Solutions Pvt. Ltd.
Mode: Online
Duration: 2 Days (6 Hours of Live Hands-on Training)
Dates: Sep 19-20, 2026
Schedule:
Session I: 5:00 PM – 6:30 PM IST
Break: 6:30 PM – 7:00 PM IST
Session II: 7:00 PM – 8:00 PM IST
🎓 Certification: E-Certificate will be provided to all participants upon successful completion of the workshop.
About the Workshop
This intensive hands-on workshop is designed to provide participants with practical training in microbiome data analysis using both Amplicon Sequencing (16S/18S/ITS) and Shotgun Metagenomics datasets. Participants will learn complete workflows from raw sequencing reads to publication-ready figures and biological interpretation.
The workshop emphasizes real-world datasets, Linux-based bioinformatics, statistical analysis, and visualization techniques widely used in microbiome research.
📅 Day 1: Amplicon Sequencing Analysis (16S/18S/ITS)
Fundamentals of microbiome studies
Amplicon sequencing workflow overview
Introduction to FASTQ files
Quality assessment using FastQC
MultiQC report generation
Primer and adapter removal
Data import into QIIME2
DADA2 denoising
ASV generation
Chimera detection and removal
Feature table creation
Representative sequence generation
Taxonomic classification
SILVA database usage
Taxonomic visualization
Relative abundance analysis
Alpha diversity analysis
Beta diversity analysis
PCoA visualization
Statistical testing
PICRUSt2 analysis
KEGG pathway prediction
Functional abundance interpretation
📅 Day 2: Shotgun Metagenomics Analysis
Applications of Shotgun Metagenomics
Experimental design considerations
FastQC
MultiQC
fastp
Host contamination removal
Kraken2 classification
Bracken abundance estimation
Krona interactive visualization
HUMAnN3 workflow
MetaPhlAn profiling
KEGG pathway analysis
Gene family abundance analysis
Assembly using MEGAHIT
Contig assessment
Introduction to MAG recovery
Taxonomic classification of assembled genomes
🛠 Software & Tools Covered
Amplicon Analysis
Linux
QIIME2
DADA2
SILVA Database
PICRUSt2
R (Phyloseq, Vegan)
Shotgun Metagenomics
FastQC
MultiQC
fastp
Kraken2
Bracken
Krona
HUMAnN3
MetaPhlAn
MEGAHIT
R Statistical Packages
🎁 Registration Benefits
✅ Live Hands-on Practical Sessions
✅ Workshop Notes & Commands Handbook
✅ Sample Datasets for Practice
✅ Installation Guide
✅ Analysis Pipelines
✅ Certificate of Participation
✅ Recording Access (90 Days)
✅ Post-Workshop Support Group
Registration Fees
The registration fee for UG and PG students is ₹899 under the Early Bird category and ₹999 under the Regular category. PhD scholars can register at ₹1,299 during the Early Bird period and ₹1,499 thereafter. The fee for faculty members is ₹1,999 for Early Bird registrations and ₹2,499 for Regular registrations. Industry professionals may participate by paying ₹2,999 under the Early Bird offer or ₹3,999 under the Regular registration category. For international participants, the registration fee is USD 65 during the Early Bird period and USD 90 for Regular registration.
Participants are encouraged to take advantage of the Early Bird offer to secure their seats at discounted rates. Seats are limited and will be allocated on a first-come, first-served basis.
Early Bird Deadline: 15 August 2026
Group Discount
5+ Registrations: 10% Discount
10+ Registrations: 15% Discount
🦠 Microbiome Data Analysis Workshop 2026
Day 1: Amplicon Sequencing Analysis (16S/18S/ITS) using QIIME2
Day 2: Shotgun Metagenomics Analysis using Kraken2, HUMAnN3 & MEGAHIT
What Participants Will Gain
On Day 1, participants will acquire practical skills in Amplicon Sequencing (16S/18S/ITS) data analysis, including sequence quality assessment, preprocessing, denoising, taxonomic classification, diversity analysis, and functional prediction using QIIME2 and PICRUSt2. By the end of the session, participants will be able to process raw sequencing data, generate microbial community profiles, perform statistical analyses, and create publication-ready visualizations for microbiome studies.
On Day 2, experience in Shotgun Metagenomics data analysis, covering quality control, taxonomic profiling, functional annotation, and assembly-based metagenomic approaches. Using tools such as Kraken2, Bracken, HUMAnN3, MetaPhlAn, and MEGAHIT, participants will learn to characterize microbial communities at species level, explore functional pathways, interpret metagenomic datasets, and derive meaningful biological insights from complex microbial ecosystems.
Upon completion of the workshop, participants will be equipped with practical knowledge, command-line skills, analysis workflows, and interpretation strategies necessary to independently perform microbiome data analysis and support research projects involving amplicon and shotgun metagenomic sequencing.
Registration
Interested participants can register using the link or QR code provided below.
Registration Link
Negenome Bio Solutions Pvt. Ltd.
📍 Corporate Office
Shernagar, Agomani, Dhubri, Assam – 783335, India
📍 Jammu Branch Office
House No. 45, Sector 3, Trikuta Nagar, Jammu, Tawi – 180012, India
📧 Email: negenome@gmail.com
📞 Phone/WhatsApp: +91 8135038866