Workshop on Microbiome Data Analysis

Workshop Title: Amplicon Sequencing (16S/18S/ITS) & Shotgun Metagenomics Data Analysis

Organized by: Negenome Bio Solutions Pvt. Ltd.
Mode: Online

Duration: 2 Days (6 Hours of Live Hands-on Training)

Dates: Sep 19-20, 2026

Schedule:

• Session I: 5:00 PM – 6:30 PM IST

• Break: 6:30 PM – 7:00 PM IST

• Session II: 7:00 PM – 8:00 PM IST

🎓 Certification: E-Certificate will be provided to all participants upon successful completion of the workshop.

About the Workshop

This intensive hands-on workshop is designed to provide participants with practical training in microbiome data analysis using both Amplicon Sequencing (16S/18S/ITS) and Shotgun Metagenomics datasets. Participants will learn complete workflows from raw sequencing reads to publication-ready figures and biological interpretation.

The workshop emphasizes real-world datasets, Linux-based bioinformatics, statistical analysis, and visualization techniques widely used in microbiome research.

📅 Day 1: Amplicon Sequencing Analysis (16S/18S/ITS)

• Fundamentals of microbiome studies

• Amplicon sequencing workflow overview

• Introduction to FASTQ files

• Quality assessment using FastQC

• MultiQC report generation

• Primer and adapter removal

• Data import into QIIME2

• DADA2 denoising

• ASV generation

• Chimera detection and removal

• Feature table creation

• Representative sequence generation

• Taxonomic classification

• SILVA database usage

• Taxonomic visualization

• Relative abundance analysis

• Alpha diversity analysis

• Beta diversity analysis

• PCoA visualization

• Statistical testing

• PICRUSt2 analysis

• KEGG pathway prediction

• Functional abundance interpretation

📅 Day 2: Shotgun Metagenomics Analysis

• Applications of Shotgun Metagenomics

• Experimental design considerations

• FastQC

• MultiQC

• fastp

• Host contamination removal

• Kraken2 classification

• Bracken abundance estimation

• Krona interactive visualization

• HUMAnN3 workflow

• MetaPhlAn profiling

• KEGG pathway analysis

• Gene family abundance analysis

• Assembly using MEGAHIT

• Contig assessment

• Introduction to MAG recovery

• Taxonomic classification of assembled genomes

🛠 Software & Tools Covered

Amplicon Analysis

• Linux

• QIIME2

• DADA2

• SILVA Database

• PICRUSt2

• R (Phyloseq, Vegan)

Shotgun Metagenomics

• FastQC

• MultiQC

• fastp

• Kraken2

• Bracken

• Krona

• HUMAnN3

• MetaPhlAn

• MEGAHIT

• R Statistical Packages

🎁 Registration Benefits

✅ Live Hands-on Practical Sessions
✅ Workshop Notes & Commands Handbook
✅ Sample Datasets for Practice
✅ Installation Guide
✅ Analysis Pipelines
✅ Certificate of Participation
✅ Recording Access (90 Days)
✅ Post-Workshop Support Group

 Registration Fees

The registration fee for UG and PG students is ₹899 under the Early Bird category and ₹999 under the Regular category. PhD scholars can register at ₹1,299 during the Early Bird period and ₹1,499 thereafter. The fee for faculty members is ₹1,999 for Early Bird registrations and ₹2,499 for Regular registrations. Industry professionals may participate by paying ₹2,999 under the Early Bird offer or ₹3,999 under the Regular registration category. For international participants, the registration fee is USD 65 during the Early Bird period and USD 90 for Regular registration.

Participants are encouraged to take advantage of the Early Bird offer to secure their seats at discounted rates. Seats are limited and will be allocated on a first-come, first-served basis.

Early Bird Deadline: 15 August 2026

Group Discount

• 5+ Registrations: 10% Discount

• 10+ Registrations: 15% Discount

🦠 Microbiome Data Analysis Workshop 2026

Day 1: Amplicon Sequencing Analysis (16S/18S/ITS) using QIIME2
Day 2: Shotgun Metagenomics Analysis using Kraken2, HUMAnN3 & MEGAHIT

What Participants Will Gain

On Day 1, participants will acquire practical skills in Amplicon Sequencing (16S/18S/ITS) data analysis, including sequence quality assessment, preprocessing, denoising, taxonomic classification, diversity analysis, and functional prediction using QIIME2 and PICRUSt2. By the end of the session, participants will be able to process raw sequencing data, generate microbial community profiles, perform statistical analyses, and create publication-ready visualizations for microbiome studies.

On Day 2, experience in Shotgun Metagenomics data analysis, covering quality control, taxonomic profiling, functional annotation, and assembly-based metagenomic approaches. Using tools such as Kraken2, Bracken, HUMAnN3, MetaPhlAn, and MEGAHIT, participants will learn to characterize microbial communities at species level, explore functional pathways, interpret metagenomic datasets, and derive meaningful biological insights from complex microbial ecosystems.

Upon completion of the workshop, participants will be equipped with practical knowledge, command-line skills, analysis workflows, and interpretation strategies necessary to independently perform microbiome data analysis and support research projects involving amplicon and shotgun metagenomic sequencing.

Registration

Interested participants can register using the link or QR code provided below.

Registration Link

https://docs.google.com/forms/d/e/1FAIpQLSc00aChGv3MzEBScqiD5SWl_bm8T1OFyqhTUar24l7dGJXgeg/viewform?usp=sharing&ouid=100782709281982599229

Negenome Bio Solutions Pvt. Ltd.

📍 Corporate Office
Shernagar, Agomani, Dhubri, Assam – 783335, India

📍 Jammu Branch Office
House No. 45, Sector 3, Trikuta Nagar, Jammu, Tawi – 180012, India

📧 Email: negenome@gmail.com

📞 Phone/WhatsApp: +91 8135038866